西亚试剂：：A novel class of tRNA-derived small RNAs extremely

Hongying Peng, Junchao Shi, Ying Zhang, He Zhang, Shangying Liao, Wei Li, Li Lei, Chunsheng Han, Lina Ning, Yujing Cao, Qi Zhou, Qi Chen and Enkui Duan

The discovery of sperm-borne RNAs (mRNAs and small non-coding RNAs) has opened the possibility of additional paternal contributions aside from providing the DNA. It has been reported that the incoming sperm can provide information for its host egg cytoplasm, which functionally influences the order of cell division, possibly via delivering RNAs. Indeed, the sperm-borne miRNA and mRNA have been demonstrated as active players in early embryo development and transgenerational epigenetic inheritance. However, given the diversity of small RNA classes (miRNA, endo-siRNA, piRNA, etc.) generated during spermatogenesis, the contents and profiles of the small RNA population carried by mature sperm remain undefined. In the present study, we isolated mature sperm from the cauda epididymis of adult male mice. The purity of sperm was > 99% as evaluated by microscopy and was confirmed by RT-PCR analyses of different biomarkers . The RNA extracted from mature sperm, adult testis, and uterus were processed for small RNA (< 40 nt) deep sequencing .

丙烯醛肉桂醛桂皮醛4-硝基肉桂醛