西亚试剂：Basement Membrane Assembly, Tissue Engineering and Stem Cel

Basement Membrane Assembly, Tissue Engineering and Stem Cells: Basement membranes are composed of large glyco-proteins such as type IV collagen, laminin, heparin sulfate proteoglycan and nidogen/entactin. In the recent years, tissue specific variants/isoforms of type IV collagen, laminin, etc have been identified, leading to the proposal of a new concept that 'not all basement membranes are created equal'. This immediately raises the question as to the function of these specific basement membranes in the regulation of cellular behavior tailored to the need to a given tissue. In the laboratory we have now isolated several tissue specific basement membranes and performed in vitro self-assembly studies. Immediate research goals for these tissue specific basement membranes include studies related to tissue engineering and propagation of stem cell cultures. Recent studies in the laboratory suggest that stem-cell therapy can be successfully used to repair matrix defects in the kidney and elsewhere.

References:
§ Zeisberg E, Tarnavksi O, Zeisberg M, Dorfman A, McMullen J, Gustafsson E, Chandraker A, Yuan X, Pu W, Roberts A, Neilson E, Sayegh M, Izumo S, Kalluri R. Endothelial to Mesenchymal Transition Contributes to Cardiac Fibrosis. Nat Med. 2007 (In Press)

§ Sugimoto H, Mundel T, Sund M, Xie L, Cosgrove D, Kalluri R. Bone Marrow Derived Stem Cells Repair Basement Membrane Collagen Defects and Reverse Genetic Kidney Disease. PNAS. 2006: 103 (19): 7321-7326.

Sund, M, Hamano Y, Sugimoto H, Sudhakar A, Soubasakos M, Yerramalla U, Benjamin L E, Lawler J, Kieran M, Shah A, and Kalluri R. The Function of Endogenous Inhibitors of Angiogenesis as Endothelium-Specific Tumor Suppressors. PNAS. 2005; 102: 2934-9.