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Common effector processing mediates cell-specific responses to stimuli
Kathryn Miller-Jensen1,2,5, Kevin A. Janes1,3,5, Joan S. Brugge3 & Douglas A. Lauffenburger1,2,4
Center for Cell Decision Processes, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
Departments of Biology and Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
These authors contributed equally to this work.
Correspondence to: Douglas A. Lauffenburger1,2,4 Correspondence and requests for materials should be addressed to D.A.L. (EmailAbstract
The fundamental components of many signalling pathways are common to all cells1, 2, 3. However, stimulating or perturbing the intracellular network often causes distinct phenotypes that are specific to a given cell type4, 5. This 'cell specificity' presents a challenge in understanding how intracellular networks regulate cell behaviour and an obstacle to developing drugs that treat signalling dysfunctions6, 7. Here we apply a systems-modelling approach8 to investigate how cell-specific signalling events are integrated through effector proteins to cause cell-specific outcomes. We focus on the synergy between tumour necrosis factor and an adenoviral vector as a therapeutically relevant stimulus that induces cell-specific responses9, 10, 11. By constructing models that estimate how kinase-signalling events are processed into phenotypes through effector substrates, we find that accurate predictions of cell specificity are possible when different cell types share a common 'effector-processing' mechanism. Partial-least-squares regression models based on common effector processing accurately predict cell-specific apoptosis, chemokine release, gene induction, and drug sensitivity across divergent epithelial cell lines. We conclude that cell specificity originates from the differential activation of kinases and other upstream transducers, which together enable different cell types to use common effectors to generate diverse outcomes. The common processing of network signals by downstream effectors points towards an important cell biological principle, which can be applied to the understanding of cell-specific responses to targeted drug therapies6.
