西亚试剂： Primary Culture of Normal Human Mammary Epithelial Cells

 Primary Culture of Normal Human Mammary Epithelial Cells

(A) Comparison of population doublings of mammary epithelial cells simultaneously cultured from organoids isolated from the same donor in WIT (blue circles) medium on Primaria plates or in MEGM (red squares) medium on regular culture plates. Cells that were cultured in MEGM growth arrested after five to six population doublings; in contrast, cells that were cultured in WIT proliferated past 40 population doublings.

(B) Comparison of p53 and p16 protein expression levels in mammary epithelial cells cultured in WIT medium on Primaria plates versus MEGM medium on regular plates on day 21, immediately prior to growth arrest of cells in MEGM medium. Western blot, β-actin loading control.

(C) Comparison of p16INK4A gene promoter methylation analysis by using DNA methylation-specific PCR primers (U, unmethylated; M, methylated; W, wild-type. p16INK4A promoter DNA-specific primers produce a single PCR product of different sizes with a complete chemical modification reaction; U primers amplify only unmethylated DNA (154 bp), M primers amplify only methylated DNA (145 bp), and W primers amplify only DNA that is not chemically modified, or “wild-type” (142 bp).

• 以上资料由西亚试剂：http://www.xiyashiji.com/ 提供此产品的详细信息如密度,含量,分子式,分子量等均可在西亚官网查询   
• 相关产品如汞乙酸汞氯化汞氧化汞碘化汞硫酸汞硝酸汞溴化汞硝酸亚汞氯化亚汞乙酸苯汞碘化汞钾硫氰酸汞氯化氨基汞三氯生三氯氧磷三氯乙烯水合氯醛三氯化磷三氯化钌三氯化钛三氯化铱三氯化铑三氯硫磷三氯乙烷三氯甲烷三氯卡班TCC1,3,5-三氯苯1,2,4-三氯苯1,2,3-三氯苯无水氯化铝三氯乙酸酐三氯乙酸钠碘甲烷二碘甲烷三碘甲烷 三氟碘甲烷硫酸二甲酯氯磺酸苯硫酚苯硫酚钠3-氨基苯硫酚2,6-二氯苯硫酚2,4-二氯苯硫酚2,5-二氯苯硫酚2-甲氧基苯硫酚2-氯乙醇 等均有销售.欢迎订购