西亚试剂：Prospective identification of myogenic endothelial cells in

Prospective identification of myogenic endothelial cells in human skeletal muscle

Bo Zheng1, Baohong Cao1, Mihaela Crisan1, Bin Sun1, Guangheng Li1, Alison Logar2, Solomon Yap1, Jonathan B Pollett1,3, Lauren Drowley1, Theresa Cassino1, Burhan Gharaibeh1, Bridget M Deasy1, Johnny Huard1,4,5 & Bruno Péault1,2,5

We document anatomic, molecular and developmental relationships between endothelial and myogenic cells within human skeletal muscle. Cells coexpressing myogenic and endothelial cell markers (CD56, CD34, CD144) were identified by immunohistochemistry and flow cytometry. These myoendothelial cells regenerate myofibers in the injured skeletal muscle of severe combined immunodeficiency mice more effectively than CD56+ myogenic progenitors. They proliferate long term, retain a normal karyotype, are not tumorigenic and survive better under oxidative stress than CD56+ myogenic cells. Clonally derived myoendothelial cells differentiate into myogenic, osteogenic and chondrogenic cells in culture. Myoendothelial cells are amenable to biotechnological handling, including purification by flow cytometry and long-term expansion in vitro, and may have potential for the treatment of human muscle disease.