西亚试剂：Rational design of memory in eukaryotic cells

Rational design of memory in eukaryotic cells

Caroline M. Ajo-Franklin1,3,4, David A. Drubin1,3, Julian A. Eskin1, Elaine P.S. Gee2, Dirk Landgraf1, Ira Phillips1,5, and Pamela A. Silver1,6

1 Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02115, USA; 2 Harvard University Program in Biophysics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA

The ability to logically engineer novel cellular functions promises a deeper understanding of biological systems. Here we demonstrate the rational design of cellular memory in yeast that employs autoregulatory transcriptional positive feedback. We built a set of transcriptional activators and quantitatively characterized their effects on gene expression in living cells. Modeling in conjunction with the quantitative characterization of the activator-promoter pairs accurately predicts the behavior of the memory network. This study demonstrates the power of taking advantage of components with measured quantitative parameters to specify eukaryotic regulatory networks with desired properties.

[Keywords: Rational design; synthetic biology; positive feedback; memory; in vivo quantitation]

Received June 22, 2007; revised version accepted July 27, 2007.
 

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