西亚试剂：Dynamic Reprogramming of Histone Acetylation and Methylatio

Dynamic Reprogramming of Histone Acetylation and Methylation in the First Cell Cycle of Cloned Mouse Embryos

Fengchao Wang , Zhaohui Kou , Yu Zhang , and Shaorong Gao *

* To whom correspondence should be addressed. E-mailhas been considered important for development of cloned embryos reconstructed by somatic cell nuclear transfer (SCNT). In the present study, dynamic reprogramming of histone acetylation and methylation modifications was investigated in the first cell cycle of cloned embryos. Our results demonstrated that part of somatic inherited lysine acetylation on core histones (H3K9, H3K14, H4K16) could be quickly deacetylated following SCNT and reacetylation occurred following activation treatment. However, acetylation marks of the other lysine residues on core histones (H4K8, H4K12) persisted in the genome of cloned embryos with only mild deacetylation occurred in the process of SCNT and activation treatment. The somatic cloned embryos established histone acetylation modifications resembled to normal embryos produced by intra-cytoplasmic sperm injection through these two different programs. Moreover, treatment of cloned embryos with histone deacetylase inhibitor, Trichostatin A (TSA), could improve the histone acetylation resembling to normal embryos and it might contribute to improved development of TSA-treated SCNT embryos. In contrast to the asymmetric histone H3K9 tri- and dimethylation present in the parental genomes of fertilized embryos, the tri- and dimethylation of H3K9 were gradually demethylated in the cloned embryos and this histone H3K9 demethylation was suggested crucial for gene activation of cloned embryos. Together, our results indicated that dynamic reprogramming of histone acetylation and methylation modifications in cloned embryos was developmentally regulated.

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