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西亚试剂：Generation of functional multipotent adult stem cells from

Generation of functional multipotent adult stem cells from GPR125+ germline progenitors

Marco Seandel1,3, Daylon James1, Sergey V. Shmelkov1, Ilaria Falciatori1, Jiyeon Kim1, Sai Chavala1, Douglas S. Scherr2, Fan Zhang1, Richard Torres5, Nicholas W. Gale5, George D. Yancopoulos5, Andrew Murphy5, David M. Valenzuela5, Robin M. Hobbs4,6, Pier Paolo Pandolfi4,6 & Shahin Rafii1

  1. Howard Hughes Medical Institute, Department of Genetic Medicine, and,
  2. Department of Urology, Weill Cornell Medical College, New York 10065, USA
  3. Division of Medical Oncology, Department of Medicine, and,
  4. Cancer Biology and Genetics Program, Department of Pathology, Memorial Sloan-Kettering Cancer Center, New York 10065, USA
  5. Regeneron Pharmaceuticals, Tarrytown, New York 10591, USA
  6. Cancer Genetics Program, Beth Israel Deaconess Cancer Center, Harvard Medical School, Boston, Massachusetts 02115, USA

Correspondence to: Shahin Rafii1 Correspondence and requests for materials should be addressed to S.R. (Email: srafii@med.cornell.edu).

Adult mammalian testis is a source of pluripotent stem cells1. However, the lack of specific surface markers has hampered identification and tracking of the unrecognized subset of germ cells that gives rise to multipotent cells2. Although embryonic-like cells can be derived from adult testis cultures after only several weeks in vitro1, it is not known whether adult self-renewing spermatogonia in long-term culture can generate such stem cells as well. Here, we show that highly proliferative adult spermatogonial progenitor cells (SPCs) can be efficiently obtained by cultivation on mitotically inactivated testicular feeders containing CD34+ stromal cells. SPCs exhibit testicular repopulating activity in vivo and maintain the ability in long-term culture to give rise to multipotent adult spermatogonial-derived stem cells (MASCs). Furthermore, both SPCs and MASCs express GPR125, an orphan adhesion-type G-protein-coupled receptor. In knock-in mice bearing a GPR125–beta-galactosidase (LacZ) fusion protein under control of the native Gpr125 promoter (GPR125–LacZ), expression in the testis was detected exclusively in spermatogonia and not in differentiated germ cells. Primary GPR125–LacZ SPC lines retained GPR125 expression, underwent clonal expansion, maintained the  of germline stem cells, and reconstituted spermatogenesis in busulphan-treated mice. Long-term cultures of GPR125+ SPCs (GSPCs) also converted into GPR125+ MASC colonies. GPR125+ MASCs generated derivatives of the three germ layers and contributed to chimaeric embryos, with concomitant downregulation of GPR125 during differentiation into GPR125- cells. MASCs also differentiated into contractile cardiac tissue in vitro and formed functional blood vessels in vivo. Molecular bookmarking by GPR125 in the adult mouse and, ultimately, in the human testis could enrich for a population of SPCs for derivation of GPR125+ MASCs, which may be employed for genetic manipulation, tissue regeneration and revascularization of ischaemic organs.