西亚试剂：we demonstrate here that MEFs infected with retroviral vect

In conclusion, we demonstrate here that MEFs infected with retroviral vectors carrying Oct4, Sox2, Myc and Klf4 are able to form morphologically distinct colonies without any selection when grown under culture condition designed for ES cells. Furthermore, a significant portion of those colonies is ES-like and positive for AP, a well known marker for embryonic stem cells. Further characterization by confocal microscopy demonstrated that iPS cells generated through this nonselective approach are positive for the established pluripotent markers such as Rex-1, Sox2 and SSEA1 (Figure 1F). We can not distinguish whether the observed Sox2 signal is due to endogenous Sox2 loci or retrovirally transduced transgene. Nevertheless, the presence of both Rex1 and SSEA1 strongly suggests that the iPS cells we obtained are similar to ES cells. While our experiments are in progress, a similar finding was reported by Meissner and colleagues7. We believe that this iPS protocol based on genetically unmodified cells should facilitate further investigations into the mechanisms of somatic cell reprogramming and the eventual application to human diseases.

References

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3. Qi H, Pei D. The magic of four: induction of pluripotent stem cells from somatic cells by Oct4, Sox2, Myc and Klf4. Cell Res 2007; 17:578–580. | Article | PubMed | ChemPort |
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• Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 2006; 126:663–676. | Article | PubMed | ISI | ChemPort |
• Wernig M, Meissner A, Foreman R, et al. In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state. Nature 2007; 448:318–324. | Article | PubMed | ISI | ChemPort |
• Okita K, Ichisaka T, Yamanaka S. Generation of germline-competent induced pluripotent stem cells. Nature 2007; 448:313–317. | Article | PubMed | ISI | ChemPort |
• Kitamura T, Koshino Y, Shibata F, et al. Retrovirus-mediated gene transfer and expression cloning:powerful tools in functional genomics. Exp Hematol 2003; 31:1007–1014. | Article | PubMed | ISI | ChemPort |
• Meissner A, Wernig M, Jaenisch R. Direct reprogramming of genetically unmodified fibroblasts into pluripotent stem cells. Nat Biotechnol 2007; 25:1177–1181. | Article | PubMed | ChemPort |
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