西亚试剂优势供应上万种化学试剂产品，欢迎各位新老客户咨询、选购！
中国
联系我们
联系方式：400-990-3999 / 邮箱：sales@xiyashiji.com
西亚试剂 —— 品质可靠，值得信赖
Human Embryonic Stem Cell Lines Generated without Embryo Destruction
Young Chung,1,6 Irina Klimanskaya,1,6 Sandy Becker,1 Tong Li,1 Marc Maserati,1 Shi-Jiang Lu,1 Tamara Zdravkovic,2 Dusko Ilic,3 Olga Genbacev,2 Susan Fisher,2,4 Ana Krtolica,3 and Robert Lanza1,5,
1 Advanced Cell Technology, Worcester, MA 01605, USA
2 Department of Cell and Tissue Biology, University of California, San Francisco, UCSF Box 0512, San Francisco, CA 94143, USA
3 StemLifeLine, San Carlos, CA 94070, USA
4 Institute for Regenerative Medicine, University of California, San Francisco School of Medicine, San Francisco, CA 94143, USA
5 Institute for Regenerative Medicine, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA
Corresponding author
Robert Lanza
To date, the derivation of all human embryonic stem cell (hESC) lines has involved destruction of embryos. We previously demonstrated that hESCs can be generated from single blastomeres (Klimanskaya et al., 2006). In that “proof-of-principle” study, multiple cells were removed from each embryo and none of the embryos were allowed to continue development. Here we report the derivation of five hESC lines without embryo destruction, including one without hESC coculture. Single blastomeres were removed from the embryos by using a technique similar to preimplantation genetic diagnosis (PGD). The biopsied embryos were grown to the blastocyst stage and frozen. The blastomeres were cultured by using a modified approach aimed at recreating the ICM niche, which substantially improved the efficiency of the hESC derivation to rates comparable to whole embryo derivations. All five lines maintained normal karyotype and markers of pluripotency for up to more than 50 passages and differentiated into all three germ layers.
