西亚试剂：Automated analysis of embryonic gene expression with cellul

Nature Methods，5, 703 - 709，John Isaac Murray，Robert H Waterston

Automated analysis of embryonic gene expression with cellular resolution in C. elegans

John Isaac Murray^1, 3, Zhirong Bao^1, 2, 3, Thomas J Boyle¹, Max E Boeck¹, Barbara L Mericle¹, Thomas J Nicholas¹, Zhongying Zhao¹, Matthew J Sandel¹ & Robert H Waterston¹

¹  Department of Genome Sciences, University of Washington School of Medicine, 1705 NE Pacific Street, Seattle, Washington 98195, USA.

²  Present address: Developmental Biology Program, Sloan-Kettering Institute, 1275 York Avenue, New York, New York 10065, USA.

³  These authors contributed equally to this work.

We describe a system that permits the automated analysis of reporter gene expression in Caenorhabditis elegans with cellular resolution continuously during embryogenesis. We demonstrate its utility by defining the expression patterns of reporters for several embryonically expressed transcription factors. The invariant cell lineage permits the automated alignment of multiple expression profiles, allowing direct comparison of the expression of different genes' reporters. We also used this system to monitor perturbations to normal development involving changes both in cell-division timing and in cell fate. Systematic application of this system could reveal the gene activity of each cell throughout development.

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