西亚试剂：Isolation and Characterization of Pluripotent Human Spermat

Isolation and Characterization of Pluripotent Human Spermatogonial Stem Cell-Derived Cells

Nina Kossack 1, Juanito Meneses 2, Shai Shefi 3, Ha Nam Nguyen 1, Shawn Chavez 1, Cory Nicholas 1, Joerg Gromoll 4, Paul J Turek 5, Renee A Reijo-Pera 1*

1 Institute for Stem Cell Biology and Regenerative Medicine; Department of Obstetrics and Gynecology; Stanford University School of Medicine, Palo Alto, CA 94304
2 Center for Reproductive Sciences and cDepartment of Urology; University of California San Francisco, San Francisco, CA 94043
3 Department of Urology; University of California San Francisco, San Francisco, CA 94043; Sheba Medical Center; Tel Hashomer 52621, Israel
4 Center of Reproductive Medicine and Andrology, University of Muenster, Domagkstrasse 11, D-48129 Muenster, Germany
5 Department of Urology; University of California San Francisco, San Francisco, CA 94043

Several reports have documented the derivation of pluripotent cells (multipotent germline stem cells (MGSCs)) from spermatogonial stem cells obtained from the adult mouse testis. These spermatogoniaderived stem cells express embryonic stem cell markers and differentiate to the three primary germ layers, as well as the germ line. Data indicates that derivation may involve reprogramming of endogenous spermatogonia in culture. Here, we report the derivation of human multipotent germline stem cells (hMGSCs) from a testis biopsy. The cells express distinct markers of pluripotency, form embryoid bodies that contain derivatives of all three germ layers, maintain a normal XY karyotype, are hypomethylated at the H19 locus and express high levels of telomerase. Teratoma assays indicate the presence of human cells 8-weeks post-transplantation but limited teratoma formation. Thus, these data suggest the potential to derive pluripotent cells from human testis biopsies but indicate a need for novel strategies to optimize hMGSC culture conditions and reprogramming.

• 以上资料由西亚试剂：http://www.xiyashiji.com/ 提供此产品的详细信息如密度,含量,分子式,分子量等均可在西亚官网查询   
• 相关产品如汞乙酸汞氯化汞氧化汞碘化汞硫酸汞硝酸汞溴化汞硝酸亚汞氯化亚汞乙酸苯汞碘化汞钾硫氰酸汞氯化氨基汞三氯生三氯氧磷三氯乙烯水合氯醛三氯化磷三氯化钌三氯化钛三氯化铱三氯化铑三氯硫磷三氯乙烷三氯甲烷三氯卡班TCC1,3,5-三氯苯1,2,4-三氯苯1,2,3-三氯苯无水氯化铝三氯乙酸酐三氯乙酸钠碘甲烷二碘甲烷三碘甲烷 三氟碘甲烷硫酸二甲酯氯磺酸苯硫酚苯硫酚钠3-氨基苯硫酚2,6-二氯苯硫酚2,4-二氯苯硫酚2,5-二氯苯硫酚2-甲氧基苯硫酚2-氯乙醇 等均有销售.欢迎订购