西亚试剂：Revascularization of ischemic limbs after transplantation o

Revascularization of ischemic limbs after transplantation of human bone marrow cells with high aldehyde dehydrogenase activity

Benjamin J. Capoccia, Debra L. Robson, Krysta D. Levac, Dustin J. Maxwell, Sarah A. Hohm, Marian J. Neelamkavil, Gillian I. Bell, Anargyros Xenocostas, Daniel C. Link, David Piwnica-Worms, Jan A. Nolta, and David A. Hess*

1 Division of Oncology, Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO, United States
2 Program in Regenerative Medicine, Krembil Centre for Stem Cell Biology, Vascular Biology Group, Robarts Research Institute, Department of Physiology and Pharmacology, University of Western Ontario, London, Ontario, Canada
3 Department of Developmental Biology, Molecular Imagaing Center, Mallinckrodt Institute of Radiology, Washington University School of Medicine, St. Louis, MO, United States
4 Division of Hematology, Department of Medicine, University of Western Ontario and the London Health Sciences Centre, London, Ontario, Canada
5 Stem Cell Program, Department of Internal Medicine, University of California, Davis, Sacramento, CA, United States

The development of cell therapies to treat peripheral vascular disease has proven difficult due to the contribution of multiple cell types that co-ordinate revascularization. Here, we characterized the vascular regenerative potential of transplanted human bone marrow (BM) cells purified by high aldehyde dehydrogenase (ALDH^hi) activity, a progenitor cell function conserved between several lineages. BM ALDH^hi cells were enriched for myelo-erythroid progenitors that produced multipotent hematopoietic reconstitution after transplantation, and contained non-hematopoietic precursors that established colonies in mesenchymal-stromal and endothelial culture conditions. The regenerative capacity of human ALDH^hi cells was assessed by intravenous transplantation into immune-deficient mice with limb ischemia induced by femoral artery ligation/transection. Compared to recipients injected with unpurified nucleated cells containing the equivalent of 2-4-fold more ALDH^hi cells, mice transplanted with purified ALDH^hi cells showed augmented recovery of perfusion and increased blood vessel density in ischemic limbs. ALDH^hi cells transiently recruited to the ischemic region but did not significantly integrate into ischemic tissue, suggesting that transient ALDH^hi cell engraftment stimulated endogenous revascularization. Thus, human BM ALDH^hi cells represent a progenitor-enriched population of several cell lineages that improves perfusion in ischemic limbs after transplantation. These clinically relevant cells may prove useful in the treatment of critical ischemia in humans.

• ^{以上资料由西亚试剂：http://www.xiyashiji.com/ 提供此产品的详细信息如密度,含量,分子式,分子量等均可在西亚官网查询}   
• ^{相关产品如汞乙酸汞氯化汞氧化汞碘化汞硫酸汞硝酸汞溴化汞硝酸亚汞氯化亚汞乙酸苯汞碘化汞钾硫氰酸汞氯化氨基汞三氯生三氯氧磷三氯乙烯水合氯醛三氯化磷三氯化钌三氯化钛三氯化铱三氯化铑三氯硫磷三氯乙烷三氯甲烷三氯卡班TCC1,3,5-三氯苯1,2,4-三氯苯1,2,3-三氯苯无水氯化铝三氯乙酸酐三氯乙酸钠碘甲烷二碘甲烷三碘甲烷 三氟碘甲烷硫酸二甲酯氯磺酸苯硫酚苯硫酚钠3-氨基苯硫酚2,6-二氯苯硫酚2,4-二氯苯硫酚2,5-二氯苯硫酚2-甲氧基苯硫酚2-氯乙醇 等均有销售.欢迎订购}