西亚试剂：Generation of induced pluripotent stem cells from human blo

Generation of induced pluripotent stem cells from human blood

Yuin-Han Loh, Suneet Agarwal, In-Hyun Park, Achia Urbach, Hongguang Huo, Garrett C. Heffner, Kitai Kim, Justine D. Miller, Kitwa Ng, and George Q. Daley*

1 Department of Medicine, Division of Pediatric Hematology Oncology, Children's Hospital Boston, and Dana-Farber Cancer Institute, Boston, MA, United States
2 Harvard Stem Cell Institute, Cambridge, MA, United States
3 Howard Hughes Medical Institute, Boston, MA, United States
4 Division of Hematology, Brigham and Women's Hospital, Boston, MA, United States

Human dermal fibroblasts obtained by skin biopsy can be reprogrammed directly to pluripotency by the ectopic expression of defined transcription factors. Here, we describe the derivation of induced pluripotent stem (iPS) cells from CD34+ mobilized human peripheral blood cells using retroviral transduction of OCT4/ SOX2/ KLF4/ MYC. Blood derived human iPS cells are indistinguishable from human embryonic stem (ES) cells with respect to morphology, expression of surface antigens and pluripotency-associated transcription factors, DNA methylation status at pluripotent cell-specific genes, and the capacity to differentiate in vitro and in teratomas. The ability to reprogram cells from human blood will allow the generation of patient-specific stem cells for diseases in which the disease-causing somatic mutations are restricted to cells of the hematopoietic lineage.

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