西亚试剂：Generation of induced pluripotent stem cell lines from tibe

Generation of induced pluripotent stem cell lines from tibetan miniature pig

Miguel Angel Esteban, Jianyong Xu, Jiayin Yang, Meixiu Peng, Dajiang Qin, Wen Li, Zhuoxin Jiang, Jiekai Chen, Kang Deng, Mei Zhong, Jinglei Cai, Liangxue Lai, and Duanqing Pei1

From the GIBH, China

Induced pluripotent stem cells (iPS) technology appears to be a general strategy to generate pluripotent stem cells from any given mammalian species. So far, iPS cells have been reported for mouse, human, rat and monkey. These four species also have established embryonic stem cell (ESC) lines which serve as gold standard for pluripotency comparisons. Attempts have been made to generate porcine ESC by various means without success. Here we report the successful generation of pluripotent stem cells from fibroblasts isolated from Tibetan miniature pig using a modified iPS protocol. The resulting iPS cell lines more closely resemble human ESC than cells from other species, have normal karyotype, stain positive for alkaline phosphatase, express high levels of ESC-like markers (Nanog, Rex1, Lin28, SSEA4), and can differentiate into teratomas composed of the three germ layers. Since porcine physiology closely resembles human, the iPS cells reported here provide an attractive model to study certain human diseases or assess therapeutical applications of iPS in a large animal model.

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