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Induction of Pluripotency in Adult Unipotent Germline Stem Cells
Kinarm Ko1,Natalia Tapia1,Guangming Wu1,Jeong Beom Kim1,Marcos J. Araúzo Bravo1,Philipp Sasse2,Tamara Glaser3,David Ruau4,5,Dong Wook Han1,Boris Greber1,Kirsten Hausd?rfer3,Vittorio Sebastiano1,Martin Stehling1,Bernd K. Fleischmann2,Oliver Brüstle3,Martin Zenke4,5andHans R. Sch?ler1,,
1 Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Münster 48149, Germany
2 Institute of Physiology I, Life & Brain Center, University of Bonn, 53105 Bonn, Germany
3 Institute of Reconstructive Neurobiology, Life & Brain Center, University of Bonn and Hertie Foundation, 53105 Bonn, Germany
4 Institute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen University Medical School, Aachen 52074, Germany
5 Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Aachen 52074, Germany
Mouse and human stem cells with features similar to those of embryonic stem cells have been derived from testicular cells. Although pluripotent stem cells have been obtained from defined germline stem cells (GSCs) of mouse neonatal testis, only multipotent stem cells have been obtained so far from defined cells of mouse adult testis. In this study we describe a robust and reproducible protocol for obtaining germline-derived pluripotent stem (gPS) cells from adult unipotent GSCs. Pluripotency of gPS cells was confirmed by invitro and invivo differentiation, including germ cell contribution and transmission. As determined by clonal analyses, gPS cells indeed originate from unipotent GSCs. We propose that the conversion process requires a GSC culture microenvironment that depends on the initial number of plated GSCs and the length of culture time.
