西亚试剂：CPAP is a cell-cycle regulated protein that controls centri

CPAP is a cell-cycle regulated protein that controls centriole length

Chieh-Ju C. Tang1, Ru-Huei Fu1,3, Kuo-Sheng Wu1,2, Wen-Bin Hsu1 & Tang K. Tang1

Centriole duplication involves the growing of a procentriole (progeny centriole) next to the proximal end of each pre-existing centriole (parental centriole). The molecular mechanisms that regulate procentriole elongation remain obscure. We show here that expression of the centriolar protein CPAP (centrosomal P4.1-associated protein) is carefully regulated during the cell cycle, with the protein being degraded in late mitosis. Depletion of CPAP inhibited centrosome duplication, whereas excess CPAP induced the formation of elongated procentriole-like structures (PLSs), which contain stable microtubules and several centriolar proteins. Ultrastructural analysis revealed that these structures are similar to procentrioles with elongated microtubules. Overexpression of a CPAP mutant (CPAP-377EE) that does not bind to tubulin dimers significantly inhibited the formation of CPAP-induced PLSs. Together, these results suggest that CPAP is a new regulator of centriole length and its intrinsic tubulin-dimer binding activity is required for procentriole elongation.

1 Institute of Biomedical Sciences, Academia Sinica, Taipei 11529, Taiwan.
2 Institute of Biochemistry and Molecular Biology, National Yang Ming University, Taipei 11221, Taiwan.
3 Current address: Graduate Institute of Immunology, China Medical University, Taichung 40402, Taiwan.

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