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Prevascularization of cardiac patch on the omentum improves its therapeutic outcome
Tal Dvira, Alon Kedemb, Emil Ruvinova, Oren Levyc, Inbar Freemana, Natalie Landad, Radka Holbovad, Micha S. Feinbergd, Shani Drore, Yoram Etzione, Jonathan Leord and Smadar Cohena,1
aDepartment of Biotechnology Engineering and
cDepartment of Life Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel 84105;
bDepartment of Obstetrics and Gynecology and
dNeufeld Cardiac Research Institute, Sheba Medical Center, Tel-Aviv University, Tel-Hashomer, Israel 52621; and
eCardiac Arrhythmia Research Laboratory, Soroka University Medical Center, Beer-Sheva, Israel 84105
The recent progress made in the bioengineering of cardiac patches offers a new therapeutic modality for regenerating the myocardium after myocardial infarction (MI). We present here a strategy for the engineering of a cardiac patch with mature vasculature by heterotopic transplantation onto the omentum. The patch was constructed by seeding neonatal cardiac cells with a mixture of prosurvival and angiogenic factors into an alginate scaffold capable of factor binding and sustained release. After 48 h in culture, the patch was vascularized for 7 days on the omentum, then explanted and transplanted onto infarcted rat hearts, 7 days after MI induction. When evaluated 28 days later, the vascularized cardiac patch showed structural and electrical integration into host myocardium. Moreover, the vascularized patch induced thicker scars, prevented further dilatation of the chamber and ventricular dysfunction. Thus, our study provides evidence that grafting prevascularized cardiac patch into infarct can improve cardiac function after MI.
