西亚试剂优势供应上万种化学试剂产品，欢迎各位新老客户咨询、选购！
中国
联系我们

登录

￥0.00

联系方式：400-990-3999 / 邮箱：sales@xiyashiji.com

西亚试剂 —— 品质可靠，值得信赖

西亚试剂：Poised Lineage Specification in Multipotential Hematopoieti

Poised Lineage Specification in Multipotential Hematopoietic Stem and Progenitor Cells by the Polycomb Protein Bmi1

Hideyuki Oguro, Jin Yuan, Hitoshi Ichikawa, Tomokatsu Ikawa, Satoshi Yamazaki, Hiroshi Kawamoto, Hiromitsu Nakauchi, Atsushi Iwama

Department of Cellular and Molecular Medicine, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan JST, CREST, Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan JST, ERATO, Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan Genetics Division, National Cancer Center Research Institute, Tokyo, 104-0045, Japan Laboratory for Lymphocyte Development, RIKEN Research Center for Allergy and Immunology, Yokohama 230-0045, Japan Laboratory of Stem Cell Therapy, Center for Experimental Medicine, Institute of Medical Sciences, University of Tokyo, Tokyo 108-8679, Japan Corresponding author

Polycomb group (PcG) proteins are essential regulators of stem cells. PcG and trithorax group proteins mark developmental regulator gene promoters with bivalent domains consisting of overlapping repressive and activating histone modifications to keep them poised for activation in embryonic stem cells. Bmi1, a component of PcG complexes, maintains the self-renewal capacity of adult stem cells, but its role in multipotency remains obscure. Here we show that Bmi1 is critical for multipotency of hematopoietic stem cells (HSCs) and multipotent progenitors (MPPs). B cell lineage developmental regulator genes, Ebf1 and Pax5, appeared to be transcriptionally repressed by bivalent domains before lineage commitment. Loss of Bmi1 resulted in a resolution of bivalent domains at the Ebf1 and Pax5 loci, leading to their premature expression in HSC/MPPs accompanied by accelerated lymphoid specification and a marked reduction in HSC/MPPs. Thus, Bmi1 is required to reinforce bivalent domains at key developmental regulator gene loci to maintain lineage specification poised for activation in adult stem cells.