西亚试剂：Amplification-free digital gene expression profiling from m

Amplification-free digital gene expression profiling from minute cell quantities
Fatih Ozsolak,David T Ting,Ben S Wittner,Brian W Brannigan,Suchismita Paul,Nabeel Bardeesy,Sridhar Ramaswamy,Patrice M Milos& Daniel A Haber

Generating reliable expression profiles from minute cell quantities is critical for scientific discovery and potential clinical applications. Here we present low-quantity digital gene expression (LQ-DGE), an amplification-free approach involving capture of poly(A)+ RNAs from cellular lysates onto poly(dT)-coated sequencing surfaces, followed by on-surface reverse transcription and sequencing. We applied LQ-DGE to profile malignant and nonmalignant mouse and human cells, demonstrating its quantitative power and potential applicability to archival specimens.

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