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Redox Regulation by Keap1 and Nrf2 Controls Intestinal Stem Cell Proliferation in Drosophila
Christine E. Hochmuth1, Benoit Biteau1, Dirk Bohmann2 and Heinrich Jasper1, ,
1 Department of Biology, University of Rochester, River Campus Box 270211, Rochester, NY 14627, USA
2 Department of Biomedical Genetics, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY 14620, USA
Highlights
? CncC, a homolog of the redox regulator Nrf2, is constitutively active in ISCs ? CncC repression by the negative regulator Keap1 is required for ISC proliferation ? Redox control by CncC/Keap1 is critical for regulating ISC proliferation ? CncC limits age-related oxidation of ISCs, maintaining intestinal homeostasis
Summary
In Drosophila, intestinal stem cells (ISCs) respond to oxidative challenges and inflammation by increasing proliferation rates. This phenotype is part of a regenerative response, but can lead to hyperproliferation and epithelial degeneration in the aging animal. Here we show that Nrf2, a master regulator of the cellular redox state, specifically controls the proliferative activity of ISCs, promoting intestinal homeostasis. We find that Nrf2 is constitutively active in ISCs and that repression of Nrf2 by its negative regulator Keap1 is required for ISC proliferation. We further show that Nrf2 and Keap1 exert this function in ISCs by regulating the intracellular redox balance. Accordingly, loss of Nrf2 in ISCs causes accumulation of reactive oxygen species and accelerates age-related degeneration of the intestinal epithelium. Our findings establish Keap1 and Nrf2 as a critical redox management system that regulates stem cell function in high-turnover tissues.
