西亚试剂：GLRs参与Ca2+内流振荡的发生

Fig. 2. GLRs are involved in the generation of Ca²⁺ influx oscillations. (A to C) Ca²⁺-specific vibrating-probe measurements recorded at the tip of tobacco pollen tubes: DSer (1 mM), CNQX (250 μM) and DNQX (250 μM)(negative flux indicates Ca²⁺ movement into pollen tube). (D) Effect on average influx (compared to non-agonist) after application of CNQX (250 mM, n = 5), DNQX (250 μM, n = 6) and D-Ser (1 mM, n = 5) application. (E) Effect of DNQX, CNQX and D-Ser, respectively on the non-oscillating flux component ("base"), oscillating flux component ("oscil.") and on the flux ratio oscil./base. CNQX and D-Ser preferentially affect the oscillating component. Error bars indicate standard deviation.

GLRs参与Ca2+内流振荡的发生。
非损伤微测技术（vibrating-probe or NMT）记录了烟草花粉管尖端的Ca²⁺流速，D-Ser (1 mM)促进了Ca²⁺内流，CNQX (250 μM)减小了Ca²⁺内流，DNQX (250 μM)减小了Ca²⁺内流。n=5-6（重复次数）。柱状图是用平均流速增加或者减少的Ca²⁺流速表示。

Fig. 3. GLR1.2 is involved in Arabidopsis pollen tube morphogenesis and in Ca²⁺ apical influx oscillations. (A) RT-PCR analysis for GLR1.2, Vanguard1 (At4g12730 - pollen specific) (20), AtEXP1 (At2g47040 absent in pollen) (20), and TUB4 expression in wild-type pollen (lane 1) and seedlings (lane 2). Lane 3 is PCR performed on genomic DNA (positive control). (B) Atglr1.2-1 insertion line showing T-DNA located within the second exon. The transcript from GLR1.2 was not detected by RT-PCR in inflorescence of Atglr1.2-1 (insert). Number of seeds per silique in wild-type, Atglr1.2-1 and GLR1.2-anti-sense plants (distribution curves, n > 100). (C) Arabidopsis wild-type pollen tube grown in the presence of CNQX (172 μM, upper panel), and Atglr1.2-1 pollen tube grown in control condition (lower panel). (D) Typical Ca²⁺-specific vibrating probe recordings in growing pollen tube of wild-type and Atglr1.2-1. (E) Effect of CNQX (172 μM) on Ca²⁺ apical influx in wild-type pollen tubes. (F) Ratio of oscillating Ca²⁺-flux in Atglr1.2-1 compared to wildtype in the presence of CNQX compared to control condition (wild-type plants).

GLR1.2参与拟南芥花粉管的形态建成和尖端Ca²⁺内流的振荡。
glr1.2-1和CNQX都导致Ca²⁺内流的减少。

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