西亚试剂：CFTR mediates bicarbonate-dependent activation of miR-125b

CFTR mediates bicarbonate-dependent activation of miR-125b in preimplantation embryo development

Yong Chao Lu1,2,3,6,*, Hui Chen1,2,*, Kin Lam Fok1,2,*, Lai Ling Tsang1,2, Mei Kuen Yu1,2, Xiao Hu Zhang1,2, Jing Chen1,2, Xiaohua Jiang1,2,4, Yiu Wa Chung1,2, Alvin Chun Hang Ma5, Anskar Yu Hung Leung5, He Feng Huang1,3 and Hsiao Chang Chan1,2,4,7

Although HCO3? is known to be required for early embryo development, its exact role remains elusive. Here we report that HCO3? acts as an environmental cue in regulating miR-125b expression through CFTR-mediated influx during preimplantation embryo development. The results show that the effect of HCO3? on preimplantation embryo development can be suppressed by interfering the function of a HCO3?-conducting channel, CFTR, by a specific inhibitor or gene knockout. Removal of extracellular HCO3? or inhibition of CFTR reduces miR-125b expression in 2 cell-stage mouse embryos. Knockdown of miR-125b mimics the effect of HCO3? removal and CFTR inhibition, while injection of miR-125b precursor reverses it. Downregulation of miR-125b upregulates p53 cascade in both human and mouse embryos. The activation of miR-125b is shown to be mediated by sAC/PKA-dependent nuclear shuttling of NF-κB. These results have revealed a critical role of CFTR in signal transduction linking the environmental HCO3? to activation of miR-125b during preimplantation embryo development and indicated the importance of ion channels in regulation of miRNAs.

• 以上资料由西亚试剂：http://www.xiyashiji.com/ 提供此产品的详细信息如密度,含量,分子式,分子量等均可在西亚官网查询   
• 相关产品如汞乙酸汞氯化汞氧化汞碘化汞硫酸汞硝酸汞溴化汞硝酸亚汞氯化亚汞乙酸苯汞碘化汞钾硫氰酸汞氯化氨基汞三氯生三氯氧磷三氯乙烯水合氯醛三氯化磷三氯化钌三氯化钛三氯化铱三氯化铑三氯硫磷三氯乙烷三氯甲烷三氯卡班TCC1,3,5-三氯苯1,2,4-三氯苯1,2,3-三氯苯无水氯化铝三氯乙酸酐三氯乙酸钠碘甲烷二碘甲烷三碘甲烷 三氟碘甲烷硫酸二甲酯氯磺酸苯硫酚苯硫酚钠3-氨基苯硫酚2,6-二氯苯硫酚2,4-二氯苯硫酚2,5-二氯苯硫酚2-甲氧基苯硫酚2-氯乙醇 等均有销售.欢迎订购