西亚试剂：Role of the repressor Oaf3p in the recruitment of transcrip

Role of the repressor Oaf3p in the recruitment of transcription factors and chromatin dynamics during oleate response

Wan Y, Arens CE, Wang S, Zuo X, Zhuo Y, Xing J.

Cellular responses to environmental stimuli are mediated by the coordinate activity of multiple control mechanisms, which result in dynamics of cell function. Communication between different levels of regulation is central for this adaptability. This study focuses on the interplay between transcriptional regulators and chromatin modifiers to cooperatively regulate transcription in response to a fatty acid stimulus. Genes involved in β-oxidation of fatty acids are highly induced in response to fatty acid exposure by four gene specific transcriptional regulators, Oaf1p, Pip2p, Oaf3p and Adr1p. Here, we examine the interplay of these factors with histone variant H2A.Z (Htz1p) in regulating the POT1 gene encoding peroxisomal thiolase, and PIP2 genes encoding the autoregulatory oleate specific transcriptional activator. Temporal resolution of ChIP data indicates that Htz1p is required for the timely removal of the transcriptional repressor Oaf3p during oleate induction. Adr1p plays an important role in the assembly of Htz1p-containing nucleosomes on the POT1 and PIP2 promoters. We also investigated the function of the uncharacterized transcriptional inhibitor, Oaf3p. Deletion of OAF3 led to faster POT1 mRNA accumulation than in the wild-type. Most impressively, a highly protected nucleosome structure on the POT1 promoter during activation was observed in the OAF3 mutant cells in response to oleate induction.

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