西亚试剂：Mice cloned from white adipose tissue-derived cells

Mice cloned from white adipose tissue-derived cells

Yiren Qin, Jiangwei Lin, Chikai Zhou, Qi Yin, Zhenfei Xie, Xuan Zhang， Xin-Yuan Liu, Weiqiang Gao and Jinsong Li

Somatic cells can be reprogrammed into totipotent or pluripotent state by nuclear transfer (NT) or the ‘Yamanaka method’. White adipose tissue (WAT) is a source of readily accessible donor cells that provide virtually unlimited cells for reprogramming. Recently, freshly isolated white adipose tissue-derived cells (ADCs) from humans or mice have been successfully reprogrammed into induced pluripotent stem cells (iPSCs) by ‘Yamanaka factors’ (Sun et al., 2009; Sugii et al., 2010). In contrast, cultured mouse ADCs were not feasible donors for NT, most likely due to chromosome instability that might occur during in vitro culture (Qin et al., 2009). Therefore, it remains unknown whether genomic stability sustains in freshly isolated mouse ADCs that can be used as donors for NT. Here, we report that cloned mice can be produced from ADCs and cloning efficiency can be dramatically improved by replacing the trophoblast cells in the cloned blastocysts with cells from tetraploid embryos. Moreover, the cloning efficiencies are consistently higher for Lin? cells than for CD45+ cells. Our results indicate that ADCs are an easily obtainable cell source that can be used as donors for studying somatic reprogramming induced by oocytes.

• 以上资料由西亚试剂：http://www.xiyashiji.com/ 提供此产品的详细信息如密度,含量,分子式,分子量等均可在西亚官网查询   
• 相关产品如诺卡氏菌液(-)-樟脑神经鞘磷脂,从牛脑所得 杨梅苷愈创奥IAPN-叔丁基苯硫腈氯化物4-溴苯基五氟化硫色胺盐酸盐4-溴噻吩-2-甲醛纤维素粉 7-(二甲基氨基)-4-三氟甲基香豆素邻硝基苯肼盐酸盐3,3'-二氨基联苯胺四盐酸盐水合物美伐他汀丁卡因阿卡明全氟辛基季胺碘化物布西拉明硫酸多粘菌素B益母草浸膏黄苓干膏 L-2,3-二氨基丙酸盐酸盐黄糊精EB替代品(GoldView)等均有销售.欢迎订购