西亚试剂：A reversible gene trap collection empowers haploid genetics

A reversible gene trap collection empowers haploid genetics in human cells

Tilmann Bürckstümmer，  Carina Banning，  Philipp Hainzl，  Richard Schobesberger，  Claudia Kerzendorfer，  Florian M Pauler，  Doris Chen，  Nicole Them，  Fiorella Schischlik，  Manuele Rebsamen，  Michal Smida，  Ferran Fece de la Cruz，  Ana Lapao，  Melissa Liszt，  Benjamin Eizinger，  Philipp M Guenzl，  Vincent A Blomen，  Tomasz Konopka，  Bianca Gapp，  Katja Parapatics，  Barbara Maier，  Johannes Stckl，  Wolfgang Fischl，  Sejla Salic，  M Rita Taba Casari

Knockout collections are invaluable tools for studying model organisms such as yeast. However， there are no large-scale knockout collections of human cells. Using gene-trap mutagenesis in near-haploid human cells， we established a platform to generate and isolate individual 'gene-trapped cells' and used it to prepare a collection of human cell lines carrying single gene-trap insertions. In most cases， the insertion can be reversed. This growing library covers 3，396 genes， one-third of the expressed genome， is DNA-barcoded and allows systematic screens for a wide variety of cellular phenotypes. We examined cellular responses to TNF-α， TGF-β， IFN-γ and TNF-related apoptosis-inducing ligand (TRAIL)， to illustrate the value of this unique collection of isogenic human cell lines.

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