西亚试剂：Genetic Incorporation of a Metal-Chelating Amino Acid as a

Genetic Incorporation of a Metal-Chelating Amino Acid as a Probe for Protein Electron Transfer

Xiaohong Liu, Jiasong Li, Jianshu Dong, Cheng Hu, Weimin Gong, Jiangyun Wang

Through the incorporation of the metal-chelating amino acid pyTyr into green fluorescent protein (GFP) from jellyfish, photoinduced electron transfer (ET) from the GFP chromophore to a bound Cu(II) ion was shown to occur within one nanosecond in a distance dependent manner. The crystal structure of GFP with pyTyr at a specific position shows the structural basis for the nanomolar binding affinity of pyTyr to Cu(II) ions.

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