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西亚试剂：Regulation of cell cycle progression and gene expression by

Regulation of cell cycle progression and gene expression by H2A deubiquitination

Heui-Yun Joo1,4, Ling Zhai1,4, Chunying Yang1, Shuyi Nie2, Hediye Erdjument-Bromage3, Paul Tempst3, Chenbei Chang2 & Hengbin Wang1

  1. Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Kaul Human Genetics Building 402A, 720 South 20th Street, Birmingham, Alabama 35294, USA
  2. Department of Cell Biology, University of Alabama at Birmingham, MCLM 360, Birmingham, Alabama 35294-0005, USA
  3. Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, New York 10021, USA
  4. These authors contributed equally to this work.

Correspondence to: Hengbin Wang1 Correspondence and requests for materials should be addressed to H.W. (Email: hbwang@uab.edu).

Post-translational histone modifications have important regulatory roles in chromatin structure and function1, 2, 3. One example of such modifications is histone ubiquitination, which occurs predominately on histone H2A and H2B. Although the recent identification of the ubiquitin ligase for histone H2A has revealed important roles for H2A ubiquitination in Hox gene silencing4, 5, 6 as well as in X-chromosome inactivation7, 8, the enzyme(s) involved in H2A deubiquitination and the function of H2A deubiquitination are not known. Here we report the identification and functional characterization of the major deubiquitinase for histone H2A, Ubp-M (also called USP16). Ubp-M prefers nucleosomal substrates in vitro, and specifically deubiquitinates histone H2A but not H2B in vitro and in vivo. Notably, knockdown of Ubp-M in HeLa cells results in slow cell growth rates owing to defects in the mitotic phase of the cell cycle. Further studies reveal that H2A deubiquitination by Ubp-M is a prerequisite for subsequent phosphorylation of Ser 10 of H3 and chromosome segregation when cells enter mitosis. Furthermore, we demonstrate that Ubp-M regulates Hox gene expression through H2A deubiquitination and that blocking the function of Ubp-M results in defective posterior development in Xenopus laevis. This study identifies the major deubiquitinase for histone H2A and demonstrates that H2A deubiquitination is critically involved in cell cycle progression and gene expression.