西亚试剂：Organization of an Activator-Bound RNA Polymerase Holoenzym

Organization of an Activator-Bound RNA Polymerase Holoenzyme

Daniel Bose1,4,Tillmann Pape1,4,Patricia C. Burrows2,Mathieu Rappas1,5,Siva R. Wigneshweraraj3,Martin Buck2andXiaodong Zhang1,,

1 Division of Molecular Biosciences, Centre for Structural Biology, Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London SW7 2AZ, UK
2 Division of Biology, Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London SW7 2AZ, UK
3 Division of Investigative Science, Department of Microbiology, Faculty of Medicine, Imperial College London, London SW7 2AZ, UK

Transcription initiation involves the conversion from closed promoter complexes, comprising RNA polymerase (RNAP) and double-stranded promoter DNA, to open complexes, in which the enzyme is able to access the DNA template in a single-stranded form. The complex between bacterial RNAP and its major variant sigma factor σ⁵⁴ remains as a closed complex until ATP hydrolysis-dependent remodeling by activator proteins occurs. This remodeling facilitates DNA melting and allows the transition to the open complex. Here we present cryoelectron microscopy reconstructions of bacterial RNAP in complex with σ⁵⁴ alone, and of RNAP-σ⁵⁴ with an AAA+ activator. Together with photo-crosslinking data that establish the location of promoter DNA within the complexes, we explain why the RNAP-σ⁵⁴ closed complex is unable to access the DNA template and propose how the structural changes induced by activator binding can initiate conformational changes that ultimately result in formation of the open complex.

• 以上资料由西亚试剂：http://www.xiyashiji.com/ 提供此产品的详细信息如密度,含量,分子式,分子量等均可在西亚官网查询   
• 相关产品如溴化汞硝酸亚汞氯化亚汞乙酸苯汞氯化钾汞 碘化汞钾硫氰酸汞硫酸亚汞氧化汞氯化汞碘化汞硝酸汞三氯氧磷三氯化磷碘甲烷二碘甲烷三碘甲烷三氟碘甲烷氘代碘甲烷碘乙烷1,2-二碘乙烷甲酸铷碘化铷溴化铷铬酸铷硫酸铷氟化铷硝酸铷氯化铷碳酸铷硫酸镱 碳酸镱氯化镱硝酸镱氧化镱等均有销售.欢迎订购