西亚试剂：Human RAP1 inhibits non-homologous end joining at telomeres

Human RAP1 inhibits non-homologous end joining at telomeres

Jay Sarthy1,2, Nancy S Bae1, Jonathan Scrafford1 and Peter Baumann1,3

1 Stowers Institute for Medical Research, Kansas City, MO, USA
2 Department of Molecular Biosciences, University of Kansas, Lawrence, KS, USA
3 Department of Molecular and Integrative Physiology, Kansas University Medical Center, Kansas City, KS, USA

Telomeres, the nucleoprotein structures at the ends of linear chromosomes, promote genome stability by distinguishing chromosome termini from DNA double-strand breaks (DSBs). Cells possess two principal pathways for DSB repair: homologous recombination and non-homologous end joining (NHEJ). Several studies have implicated TRF2 in the protection of telomeres from NHEJ, but the underlying mechanism remains poorly understood. Here, we show that TRF2 inhibits NHEJ, in part, by recruiting human RAP1 to telomeres. Heterologous targeting of hRAP1 to telomeric DNA was sufficient to bypass the need for TRF2 in protecting telomeric DNA from NHEJ in vitro. On expanding these studies in cells, we find that recruitment of hRAP1 to telomeres prevents chromosome fusions caused by the loss of TRF2/hRAP1 from chromosome ends despite activation of a DNA damage response. These results provide the first evidence that hRAP1 inhibits NHEJ at mammalian telomeres and identify hRAP1 as a mediator of genome stability.

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