西亚试剂：Innate immune and chemically triggered oxidative stress mod

Innate immune and chemically triggered oxidative stress modifies translational fidelity

Nir Netzer1,6, Jeffrey M. Goodenbour2,6, Alexandre David1, Kimberly A. Dittmar3, Richard B. Jones4, Jeffrey R. Schneider5, David Boone5, Eva M. Eves4, Marsha R. Rosner4, James S. Gibbs1, Alan Embry1, Brian Dolan1, Suman Das1, Heather D. Hickman1, Peter Berglund1, Jack R. Bennink1, Jonathan W. Yewdell1,6 & Tao Pan3,6

1 Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892, USA
2 Department of Human Genetics,
3 Department of Biochemistry and Molecular Biology,
4 Ben May Institute,
5 Department of Medicine, University of Chicago, Chicago, Illinois 60637, USA
6 These authors contributed equally to this work.
Correspondence to: Jonathan W. Yewdell1,6Tao Pan3,6 Correspondence and requests for materials should be addressed to J.W.Y. or T.P.

Translational fidelity, essential for protein and cell function, requires accurate transfer RNA (tRNA) aminoacylation. Purified aminoacyl-tRNA synthetases exhibit a fidelity of one error per 10,000 to 100,000 couplings1, 2. The accuracy of tRNA aminoacylation in vivo is uncertain, however, and might be considerably lower3, 4, 5, 6. Here we show that in mammalian cells, approximately 1% of methionine (Met) residues used in protein synthesis are aminoacylated to non-methionyl-tRNAs. Remarkably, Met-misacylation increases up to tenfold upon exposing cells to live or non-infectious viruses, toll-like receptor ligands or chemically induced oxidative stress. Met is misacylated to specific non-methionyl-tRNA families, and these Met-misacylated tRNAs are used in translation. Met-misacylation is blocked by an inhibitor of cellular oxidases, implicating reactive oxygen species (ROS) as the misacylation trigger. Among six amino acids tested, tRNA misacylation occurs exclusively with Met. As Met residues are known to protect proteins against ROS-mediated damage7, we propose that Met-misacylation functions adaptively to increase Met incorporation into proteins to protect cells against oxidative stress. In demonstrating an unexpected conditional aspect of decoding mRNA, our findings illustrate the importance of considering alternative iterations of the genetic code.

• 以上资料由西亚试剂：http://www.xiyashiji.com/ 提供此产品的详细信息如密度,含量,分子式,分子量等均可在西亚官网查询   
• 相关产品如溴化汞硝酸亚汞氯化亚汞乙酸苯汞氯化钾汞 碘化汞钾硫氰酸汞硫酸亚汞氧化汞氯化汞碘化汞硝酸汞三氯氧磷三氯化磷碘甲烷二碘甲烷三碘甲烷三氟碘甲烷氘代碘甲烷碘乙烷1,2-二碘乙烷甲酸铷碘化铷溴化铷铬酸铷硫酸铷氟化铷硝酸铷氯化铷碳酸铷硫酸镱 碳酸镱氯化镱硝酸镱氧化镱等均有销售.欢迎订购