西亚试剂：Encoded Multichromophore Response for Simultaneous Label-Fr

Encoded Multichromophore Response for Simultaneous Label-Free Detection
Constantin Pistol 1, Vincent Mao 1, Viresh Thusu 1, Alvin R. Lebeck 2, Chris Dwyer 1 *

1Department of Electrical and Computer Engineering Duke University 130 Hudson Hall Durham, NC 27708 (USA)
2Department of Computer Science Duke University Box 90921, Durham, NC 27708 (USA)

The self-assembly of molecularly precise nanostructures is widely expected to form the basis of future high-speed integrated circuits, but the technologies suitable for such circuits are not well understood. In this work, DNA self-assembly is used to create molecular logic circuits that can selectively identify specific biomolecules in solution by encoding the optical response of near-field coupled arrangements of chromophores. The resulting circuits can detect label-free, femtomole quantities of multiple proteins, DNA oligomers, and small fragments of RNA in solution via ensemble optical measurements. This method, which is capable of creating multiple logic-gate-sensor pairs on a 2 × 80 × 80-nm DNA grid, is a step toward more sophisticated nanoscale logic circuits capable of interfacing computers with biological processes.

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