西亚试剂：Chemical synthesis of the mouse mitochondrial genome

Chemical synthesis of the mouse mitochondrial genome
Daniel G Gibson,dgibson@jcvi.orgHamilton O Smith,Clyde A Hutchison III,J Craig Venter& Chuck Merryman

We describe a one-step, isothermal assembly method for synthesizing DNA molecules from overlapping oligonucleotides. The method cycles between in vitro recombination and amplification until the desired length is reached. As a demonstration of its simplicity and robustness, we synthesized the entire 16.3-kilobase mouse mitochondrial genome from 600 overlapping 60-mers.

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