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Rice is sensitive to cold and can be grown only in certain climate zones. Human selecion ofjaponica rice has extended its growth zone to regions with lower temperature, while the molecular basis of this adaptation remains unknown. Here, we identify the quantitative trait locusCOLD1 that confers chilling tolerance in japonica rice. Overexpression of COLD1jap significantly enhances chilling tolerance, wheras rice lines with deficiency or downregulation of COLD1japare sensitive to cold. COLD1 encodes a regulator of G-protein signaling that localizes on plasma membrane and endoplasmic reticulum (ER). It interacts with the G-protein α subunit to activate the Ca2+ channel for sensing low temperature and to accelerate G-protein GTPase activity. We further identify that a SNP in COLD1, SNP2, originated from Chinese Oryza rufipogon, is responsible for the ability of COLDjap/ind to confer chilling tolerance, supporting the importance of COLD1 in plant adaptation.以上资料由西亚试剂:http://www.xiyashiji.com/