西亚试剂：Next-generation sequencing to generate interactome datase

Next-generation sequencing to generate interactome datase

Haiyuan Yu; Leah Tardivo; Stanley Tam; Evan Weiner; Fana Gebreab; Changyu Fan; Nenad Svrzikapa; Tomoko Hirozane-Kishikawa; Edward Rietman; Xinping Yang; Julie Sahalie; Kourosh Salehi-Ashtiani; Tong Hao; Michael E Cusick; David E Hill; Frederick P Roth; Pascal Braun; Marc Vidal

Next-generation sequencing has not been applied to protein-protein interactome network mapping so far because the association between the members of each interacting pair would not be maintained in en masse sequencing. We describe a massively parallel interactome-mapping pipeline, Stitch-seq, that combines PCR stitching with next-generation sequencing and used it to generate a new human interactome dataset. Stitch-seq is applicable to various interaction assays and should help expand interactome network mapping.