西亚试剂：Tet Proteins Can Convert 5- Methylcytosine

Tet Proteins Can Convert 5- Methylcytosine to 5-Formylcytosine and 5- Carboxylcytosine

John M. Dawes, Margarita Calvo, James R. Perkins, Kathryn J. Paterson, Hannes Kiesewetter, Carl Hobbs, Timothy K. Y. Kaan, Christine Orengo, David L. H. Bennett and Stephen B. McMahon

The species boundaries of some venerids are difficult to define based solely on morphological features due to their indistinct intra- and interspecific phenotypic variability. An unprecedented biodiversity crisis caused by human activities has emerged. Thus, to access the biological diversity and further the conservation of this taxonomically muddling bivalve group, a fast and simple approach that can efficiently examine species boundaries and highlight areas of unrecognized diversity is urgently needed. DNA barcoding has proved its effectiveness in high-volume species identification and discovery. In the present study, Chinese fauna was chosen to examine whether this molecular biomarker is sensitive enough for species delimitation, and how it complements taxonomy and explores species diversity.The present study shows that DNA barcoding is effective in species delimitation and can aid taxonomists by indicating useful diagnostic morphological traits, informing needful revision, and flagging unseen species. Moreover, the BOLD system, which deposits barcodes, morphological, geographical and other data, has the potential as a convenient taxonomic platform