西亚试剂：Gene–protein correlation in single cells

Gene–protein correlation in single cells

Jaclyn J. Renfrow, Adrienne C. Scheck, Neil S. Dhawan, Paul J. Lukac, Hannes Vogel, James P. Chandler, Jeffrey J. Raizer, Griffith R. Harsh, Arnab Chakravarti and Markus Bredel

We present a novel methodology combining traditional fluorescent in situ hybridization with an in situ protein detection technology called proximity ligation assay. This method has potential to perform a detailed analysis of the relationship between gene status and corresponding protein expression in cells and tissues. We demonstrate that the fluorescent in situ gene protein assay methodology is capable of resolving gene and protein patterns simultaneously on a cell-by-cell basis.