西亚试剂：analysis of RNA-Seq data reveals extensive

Comprehensive analysis of RNA-Seq data reveals extensive RNA editing in a human transcriptome

Zhiyu Peng,1, 5 Yanbing Cheng,1, 5 Bertrand Chin-Ming Tan,2, 5 Lin Kang,1 Zhijian Tian,1 Yuankun Zhu,1 Wenwei Zhang,1 Yu Liang,1 Xueda Hu,1 Xuemei Tan,1 Jing Guo,1 Zirui Dong,1 Yan Liang,1 Li Bao1 & Jun Wang1, 3, 4

RNA editing is a post-transcriptional event that recodes hereditary information. Here we describe a comprehensive profile of the RNA editome of a male Han Chinese individual based on analysis of ~767 million sequencing reads from poly(A)+, poly(A)− and small RNA samples. We developed a computational pipeline that carefully controls for false positives while calling RNA editing events from genome and whole-transcriptome data of the same individual. We identified 22,688 RNA editing events in noncoding genes and introns, untranslated regions and coding sequences of protein-coding genes. Most changes (~93%) converted A to I(G), consistent with known editing mechanisms based on adenosine deaminase acting on RNA (ADAR). We also found evidence of other types of nucleotide changes; however, these were validated at lower rates. We found 44 editing sites in microRNAs (miRNAs), suggesting a potential link between RNA editing and miRNA-mediated regulation. Our approach facilitates large-scale studies to profile and compare editomes across a wide range of samples