西亚试剂：Multiplexed aberration measurement for deep tissue imaging

Chen Wang, Rui Liu, Daniel E Milkie, Wenzhi Sun, Zhongchao Tan, Aaron Kerlin, Tsai-Wen Chen, Douglas S Kim & Na Ji

We describe an adaptive optics method that modulates the intensity or phase of light rays at multiple pupil segments in parallel to determine the sample-induced aberration. Applicable to fluorescent protein–labeled structures of arbitrary complexity, it allowed us to obtain diffraction-limited resolution in various samples in vivo. For the strongly scattering mouse brain, a single aberration correction improved structural and functional imaging of fine neuronal processes over a large imaging volume.

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