磷酸二酯酶I来源于东部菱背响尾蛇毒液

别名：磷酸二脂酶产品编号：273036 CAS号：9025-82-5

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订货信息

订货编号	产品名称	规格	包装	价格	数量	操作
A61038-100UN	磷酸二酯酶I来源于东部菱背响尾蛇毒液	≥20 units/mg dry weight	100UN	1434.00

化学性质

属性

存贮条件

储存温度 -20°C

描述

产品介绍

Venom exonuclease (Phosphodiesterase I) successively hydrolyzes 5'-mononucleotides from 3'-OH-terminated ribo- and deoxyribo-oligonucleotides. The enzyme has an optimal pH range of 9.8-10.4 and a molecular weight of 115 kDa. Phosphodiesterase is inhibited by reducing agents such as glutathione, cysteine and ascorbic acids. It is completely inhibited by 5mM EDTA while ATP, ADP and AMP are partial inhibitors. The enzyme has an absolute requirement for Mg2 .

别名

磷酸二脂酶I;5′-Exonuclease;Oligonucleate 5′-nucleotidohydrolase

生化机理

Phosphodiesterase I breaks phosphodiester bonds and catalyzes the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is released from eucaryotic plasma membranes by phosphatidylinositol-specific phospholipase C.

备注

Purified by the method of Williams, Sung and Laskowski, JBC, 236, 1130 (1961). Further treated to inactivate contaminating 5'-nucleotidase activity according to Sulkowski and Laskowski: BBA, 240, 443 (1961). Lyophilized in vials.

应用

Phosphodiesterase (PDE) is any enzyme that is used to breaks phosphodiester bonds. It is a membrane-bound glycoprotein that is used to catalyze the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is used in phosphodiesterase activation assays to hydrolyze AMP.

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危险属性