半乳糖氧化酶

属性





敏感性


对湿度敏感




存贮条件


储存温度 -20°C





描述





产品介绍


Galactose oxidase oxidizes galactose and some galactose derivatives in both free and polymeric forms. Oxidation occurs at the C6 position. The enzyme has a molecular weight of 68 ± 3 kDa, and the optimum pH is 7.0.




别名


半乳糖氧化酶;D-Galactose:oxygen 6-oxidoreductase




生化机理


Galactose oxidase catalyzes the coversion of D-galactose to D-galacto-hexodialdose. 2-Deoxy-D-galactose, lactose, melibiose, raffinose and stachyose react with galactose oxidase in the peroxidase:o-tolidine system.Essentially no oxidation of D-glucose, L-galactose, L-arabinose or D-glucuronate has been observed.




备注


Supplied as a lyophilized powder containing sodium phosphate and sucrose as stabilizers.




应用


Useful in the determination of lactose.



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储存条件 ：

-20° C密闭，避光，通风干燥处

稳定性 ：

Application Useful in the determination of lactose.

Preparation Note Chromatographically purified

Unit Definition One unit will produce a ΔA425 of 1.0 per min at pH 6.0 at 25 ° C, in a peroxidase and o-tolidine system. Reaction volume = 3.4 mL. Light path = 1 cm.

Physical form Lyophilized, contains buffer salts and stabilizer

Biochem/physiol Actions 2-Deoxy-D-galactose, lactose, melibiose, raffinose and stachyose react with galactose oxidase in the peroxidase:o-tolidine system.

Essentially no oxidation of D-glucose, L-galactose, L-arabinose or D-glucuronate has been observed.



更多 ：

1. 性状：冻干粉末。

2. 密度（g/mL,25℃）：未确定

3. 相对蒸汽密度（g/mL,空气=1）：未确定

4. 熔点（℃）：未确定

5. 沸点（℃,常压）：未确定

6. 沸点（℃,5.2kPa）：未确定

7. 折射率：未确定

8. 闪点（℃）：未确定

9. 比旋光度（º ）：未确定

10. 自燃点或引燃温度（℃）：未确定

11. 蒸气压（kPa,20℃）：未确定

12. 饱和蒸气压（kPa,60℃）：未确定

13. 燃烧热（KJ/mol）：未确定

14. 临界温度（℃）：未确定

15. 临界压力（KPa）：未确定

16. 油水（辛醇/水）分配系数的对数值：未确定

17. 爆炸上限（%,V/V）：未确定

18. 爆炸下限（%,V/V）：未确定

19. 溶解性：溶于水

WGK德国


3



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危险品运输编码 ：NONH for all modes of transport