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甲氧基PEG戊二酸N-羟基琥珀酰亚胺-西亚试剂有售,甲氧基PEG戊二酸N-羟基琥珀酰亚胺分子式,甲氧基PEG戊二酸N-羟基琥珀酰亚胺价格,西亚试剂有各种化学试剂,生物试剂,分析试剂,材料试剂,高端化学,耗材,实验室试剂,科研试剂,色谱耗材www.xiyashiji.com

甲氧基PEG戊二酸N-羟基琥珀酰亚胺

别名:甲氧基PEG戊二酸N-羟基琥珀酰亚胺 产品编号:253559 分子式: 分子量: 

订货信息

订货编号 产品名称 规格 包装 价格 数量 操作
A55523-1g 甲氧基PEG戊二酸N-羟基琥珀酰亚胺 MW 2000 Da 1g 820.00

B55523-5g 甲氧基PEG戊二酸N-羟基琥珀酰亚胺 MW 5000 Da 5g 7822.00

B55523-250mg 甲氧基PEG戊二酸N-羟基琥珀酰亚胺 MW 5000 Da 250mg 541.00

化学性质

属性溶解性Off-white/white solid or viscous liquid depends on molecule weight;Soluble in regular aqeous solution as well as most organic solvents;存贮条件储存温度-20°C描述产品介绍N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol (PEG-NHS) is an amino (-NH2) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with their available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl carbonate (SC) functionalized mPEG-NHS offers superior reactivity and higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.别名;生化机理Generally, a 10 to 50-fold molar excess of NHS PEG over the amount of amine-containing material results in sufficient conjugation.Materials Required:Conjugation buffer: Sodium bicarbonate 100 mM buffer, pH 8.5 or other amine-free buffer at pH 7-8.5.PEG NHS stock solution: 100 mg in 1 mL conjugation buffer.Washing solution: Distilled water or any aqueous buffer.Reaction Steps:Dissolve targeted materials in conjugation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add NHS PEG stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of PEG NHS needed for optimal conjugation; Allow mixture agitates at room temperature for 30~60 min at room temperature or 2 hours at 4 0C. Conjugates can be purified either by size exclusion chromatography or dialysis.

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