质量标准
Descriptions
AnalysisNoteAgglutinationactivityisexpressedinμg/mlandisdeterminedfromserialdilutionsofa1mg/mlsolution.Thisactivityisthelowestconcentrationtoagglutinateasuspensionof2%humanerythrocytesinphosphatebufferedsaline,pH6.8after1hrincubationat25C.MitogenicactivityisdeterminedbyBrdUincorporationinHeLacellcultures.
Biochem/physiolActionsPHAconsistsoftwomolecularspecies,anerythroagglutinin(PHA-E)whichhaslowmitogenicactivityandhigherythroagglutininactivity,andleucoagglutinin(PHA-L)whichhashighmitogenicandleucoagglutinatingactivity,butverylowerythroagglutinatingactivity.
PHA-Eisnotbloodgroupspecific,butagglutinationcanbeinhibitedbycertainoligosaccharides.3PHA-PistheproteinformofPHApriortoseparationandpurificationoferythroagglutininandleucoagglutinin.4PHA-Misthemucoproteinform.4Conjugatesarepreparedfromthecorrespondingpurifiedlectins.
Properties
formlyophilizedpowder(containsbuffersaltsandNaCl)
potency≤10μgpermLmitogenicactivity
colorwhiteoroff-white
solubilityPBS:5mg/mLpH6.8,cleartoslightlyhazy,colorlesstofaintlyyellow
suitabilitycellculturetested
storagetemp.2-8°C